Functional genomics of cell morphology using RNA interference: pick your style, broad or deep
نویسنده
چکیده
Several new studies have used RNA interference to screen for protein functions affecting cell shape, mitosis and cytokinesis of Drosophila cells in culture. One broad survey of nearly 1,000 proteins and three studies focused on cytoskeletal and motor proteins have identified key proteins essential for these processes in animal cells.
منابع مشابه
Knocking down the obstacles to functional genomics data sharing
This week, Scientific Data published a collection of eight papers that describe datasets from high-throughput functional genomics screens, primarily utilizing RNA interference (RNAi). The publications explore host-pathogen dependencies, innate immune response, disease pathways, and cell morphology and motility at the genome-level. All data, including raw images from the high content screens, ar...
متن کاملRNA interference screens to uncover membrane protein biology.
In this review, we discuss the use of RNA interference screens to identify genes involved in the regulation and function of membrane proteins. Briefly, cells expressing the membrane protein of interest can be transduced with a pooled lentiviral short-hairpin RNA (shRNA) library containing tens of thousands of unique shRNAs. Transduced cells are then selected or fractionated based on specific cr...
متن کاملFunctional Genomics in Trypanosomes: Using RNA Interference to Assign Gene Function and Validate Drug Targets in Tropical Disease Agents
Trypanosomes are unicellular protozoa and members of the order kinetoplastida, a highly divergent branch of the eukaryota which probably separated from the mammalian lineage ~3 x 10 years ago. Pathogenic trypanosomatids include the causative agents for Chagas’ disease or South American trypanosomiasis and Kala Azar or Leishmaniasis. They are equally divergent from the Apicomplexan protozoa that...
متن کاملA transient RNA interference assay system using Arabidopsis protoplasts.
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Intr...
متن کاملStudying gene function in Caenorhabditis elegans using RNA-mediated interference.
The RNA interference (RNAi) method for targeted gene silencing is widely used in Caenorhabditis elegans for large-scale functional genomic studies, analysis of limited gene sets and detailed analysis of individual gene function. The application of RNAi has identified genes that participate in various aspects of development, physiology and cell biology. In addition, RNAi has been used to identif...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of Biology
دوره 2 شماره
صفحات -
تاریخ انتشار 2003